GENETIC TRANSFORMATION OF COMMON BEANS (Phaseolus vulgaris L.) CALLI BY BIOLISTIC GUN AND VIA Agrobacterium tumefaciens
نویسندگان
چکیده
منابع مشابه
Diversification and Population Structure in Common Beans (Phaseolus vulgaris L.)
Wild accessions of crops and landraces are valuable genetic resources for plant breeding and for conserving alleles and gene combinations in planta. The primary genepool of cultivated common beans includes wild accessions of Phaseolus vulgaris. These are of the same species as the domesticates and therefore are easily crossable with cultivated accessions. Molecular marker assessment of wild bea...
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Common bean is the most important legume worldwide for direct human consumption. Beans constitute an important source of dietary protein for over half a billion people mainly in developing countries. A great number of bacterial, fungal, and viral diseases occur annually in bean-producing regions all over the world, causing economic losses to bean producers. Major improvements in agronomic trait...
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Biolistic gun-mediated transformation is one of the two most effective and popular methods for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature e...
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Transgenic Robinia pseudoacacia plants were obtained by Agrobacterium tumefaciens mediated gene transfer. Agrobacterium strain LBA4404 harbouring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and beta-glucuronidase (GUS) genes was co-cultivated with hypocotyl segments of in vitro raised seedlings of Robinia. Parameters important for high efficiency regenerat...
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BACKGROUND White-rot fungi are primarily the major degraders of lignin, a major obstacle for commercial exploitation of plant byproducts to produce bioethanol and other industrially important products. However, to improve their efficacy for lignin degradation, it has become necessary to genetically modify these organisms using appropriate vectors. Agrobacterium tumefaciens, a soil phytopathogen...
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ژورنال
عنوان ژورنال: Egyptian Journal of Genetics and Cytology
سال: 2013
ISSN: 0046-161X
DOI: 10.21608/ejgc.2013.10454